Modulating the interaction between zo-2/tjp2 and a snail zinc finger transcription factor family member
AssigneeAgency for Science, Technology and Research
InventorsWalter Hunziker, Choon Peng Goh
Life of this patent
Estimating the term…
Abstract
There is provided a method of identifying candidate agents capable of modulating interaction between a first polypeptide and a second polypeptide, wherein the first polypeptide is ZO-2/TJP2 or a functional variant thereof and the second polypeptide is a Snail zinc finger transcription factor family member or a functional variant thereof.
Claims25
A method of identifying candidate agents capable of modulating interaction between a first polypeptide and a second polypeptide, wherein the first polypeptide is ZO-2/TJP2 or a functional variant thereof and the second polypeptide is a Snail zinc finger transcription factor family member or a functional variant thereof, the method comprising:
- a. contacting the first polypeptide with the second polypeptide and a candidate agent;
- and
- b. determining whether the binding of the first polypeptide with the second polypeptide is decreased or increased in the presence of said candidate agent when compared with a control.
A method of predicting the likelihood of tumor metastasis development or the effectiveness of a cancer treatment, the method comprising:
- determining a change in the binding of a first polypeptide with a second polypeptide when compared with a control;
- wherein the first polypeptide is ZO-2/TJP2 or a functional variant thereof and the second polypeptide is a Snail zinc finger transcription factor family member or a functional variant thereof.
A kit for identifying candidate agents capable of modulating the interaction between a first polypeptide and a second polypeptide, wherein the first polypeptide is ZO-2/TJP2 or a functional variant thereof and the second polypeptide is a Snail zinc finger transcription factor family member or a functional variant thereof, the kit comprising the first polypeptide, the second polypeptide and at least one candidate agent.
A method of treating, inhibiting or suppressing tumor growth comprising:
- administering an agent which decreases the binding of a first polypeptide and a second polypeptide,
- wherein the first polypeptide is ZO-2/TJP2 or a functional variant thereof and the second polypeptide is a Snail zinc finger transcription factor family member or a functional variant thereof.
A method of inhibiting tumor metastasis comprising:
- administering an agent which decreases the binding of a first polypeptide and a second polypeptide,
- wherein the first polypeptide is ZO-2/TJP2 or a functional variant thereof and the second polypeptide is a Snail zinc finger transcription factor family member or a functional variant thereof.
A method of enhancing wound healing comprising:
- administering an agent which increases the binding of a first polypeptide and a second polypeptide,
- wherein the first polypeptide is ZO-2/TJP2 or a functional variant thereof and the second polypeptide is a Snail zinc finger transcription factor family member or a functional variant thereof.
An isolated complex between ZO-2/TJP2 and a Snail zinc finger transcription factor family member.
(canceled)
An antibody directed against the binding portion of ZO-2/TJP2 and SNAI2 (Slug) at the guanylate kinase (GUK) domain of ZO-2/TJP2.
Dependencies are inferred from the claim text.