Phage display system
OwnerRoderick Slavcev, Jessica Nicastroinventors
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Abstract
A phage display system is provided comprising a mutant phage-infected host cell adapted to express a peptide of interest fused to a phage capsid protein, wherein the mutant phage includes a nonsense mutation which prevents expression of the capsid protein as a functional protein, and wherein expression of the peptide of interest is controlled by an inducible repressor and by a suppressor that suppresses the nonsense mutation.
Claims20
A phage display system comprising mutant phage-infected host cells adapted to express a peptide of interest fused to a phage capsid protein, wherein the mutant phage includes a nonsense mutation which prevents expression of the capsid protein as a functional protein, and wherein expression of the peptide of interest fused to the capsid protein is controlled by an inducible repressor and by a suppressor that suppresses the nonsense mutation.
A method of expressing a peptide of interest in a host cell comprising the steps of:
- 1) introducing a mutant phage adapted to express the peptide fused to a phage capsid protein to the host cell, wherein the phage includes a nonsense mutation which prevents expression of the capsid protein as a functional protein, and wherein expression of the peptide of interest is controlled by an inducible repressor and by a suppressor that suppresses the nonsense mutation, and 2) culturing the host cell under conditions that permit expression of the peptide of interest.
Dependencies are inferred from the claim text.