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AssigneeKowa Company, Ltd.
InventorKatsumi Yabusaki
Estimating the term…
Provided is a technique which can detect a physiologically active substance of biological origin or measure the concentration of the physiologically active substance of biological origin with higher accuracy or within a shorter period even when a sample that contains the physiologically active substance of biological origin at an extremely low concentration or a sample that contains a component capable of interfering with an AL is used. Microparticles capable of adsorbing a specific physiologically active substance, e.g., an endotoxin, is dispersed in a sample containing the specific physiologically active substance to cause the adsorption of the specific physiologically active substance onto the microparticles, thereby concentrating the specific physiologically active substance in the sample. Subsequently, the microparticles are separated and collected, then are reacted with an AL to cause an aggregation reaction, and the aggregation is detected by an optical means. In this manner, the measurement of the specific physiologically active substance can be carried out.
A method for measuring a physiologically active substance of biological origin by producing a mixed solution of an AL reagent comprising AL which is a hemocyte extract of limulus and a sample comprising a predetermined physiologically active substance of biological origin, and detecting protein aggregation or gelation induced by a reaction between AL and the physiologically active substance in the mixed solution with an optical technique while stirring the mixed solution, wherein detecting the physiologically active substance in the sample or measuring the concentration of the physiologically active substance comprises:
Dependencies are inferred from the claim text.