miRNA Biomarkers For Ulcerative Colitis
AssigneeGenisphere, LLC
InventorsRobert C. Getts, Thomas D. Stamato, Radharani Duttagupta, Keith W. Jones
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Abstract
Methods for diagnosing inflammatory bowel disease and ulcerative colitis using miRNA biomarkers for these diseases are provided. Differential expression of the miRNA biomarkers in blood fractions, e.g., platelets, of diseased individuals as compared to expression levels in normal individuals indicates the presence of IBD or ulcerative colitis. Also provided are microarrays for use in the diagnostic methods, wherein the features of the microarray consist essentially of nucleic acid sequences that hybridize to the miRNA biomarkers and normalization controls.
Claims18
A method for diagnosing inflammatory bowel disease comprising:
- a) quantifying individual miRNAs in a sample derived from fractionated blood samples,
- b) comparing the quantity of each quantified miRNA in the sample with a reference quantity representing normal expression levels of the miRNA;
- and
- c) identifying miRNAs which are differentially expressed in the sample compared to normal expression levels, wherein an increase or decrease in expression of at least 1.4-fold in at least one miRNA compared to normal expression levels indicates IBD.
A microarray comprising miRNA features for diagnosis of ulcerative colitis, wherein the miRNA features comprise:
- a) pl-hsa-let-7i-star_st, pl-hsa-miR-1271_st, pl-hsa-miR-1274b_st, pl-hsa-miR-1296_st, pl-hsa-miR-138_st, pl-hsa-miR-140-3p_st, pl-hsa-miR-143-star_st, pl-hsa-miR-146b-3p_st, pl-hsa-miR-150-star_st, pl-hsa-miR-18 lb_st, pl-hsa-miR-188-5p_st, pl-hsa-miR-20b-star_st, pl-hsa-miR-22_st, pl-hsa-miR-27a-star_st, pl-hsa-miR-31_st, pl-hsa-miR-330-3p_st, pl-hsa-miR-345_st, pl-hsa-miR-362-5p_st, pl-hsa-miR-378_st, pl-hsa-miR-378-star_st, pl-hsa-miR-422a_st, pl-hsa-miR-500_st, pl-hsa-miR-501-5p_st, pl-hsa-miR-532-5p_st, pl-hsa-miR-550-star_st, pl-hsa-miR-720_st, pl-hsa-miR-769-3p_st, pl-hsa-miR-769-5p_st, pl-hsa-miR-874_st, pl-hsa-miR-92a-1-star_st, and pl-hsa-miR-941_st;
- and/or
- b) pl-hsa-miR-1274b_st, pl-hsa-miR-138_st, pl-hsa-miR-140-3p_st, pl-hsa-miR-143-star_st, pl-hsa-miR-146b-3p_st, pl-hsa-miR-150-star_st, pl-hsa-miR-181b_st, pl-hsa-miR-188-5p_st, pl-hsa-miR-20b-star_st, pl-hsa-miR-22_st, pl-hsa-miR-27a-star_st, pl-hsa-miR-330-3p_st, pl-hsa-miR-362-5p_st, pl-hsa-miR-378_st, pl-hsa-miR-378-star_st, pl-hsa-miR-422a_st, pl-hsa-miR-423-3p_st, pl-hsa-miR-500_st, pl-hsa-miR-501-5p_st, pl-hsa-miR-532-5p_st, pl-hsa-miR-550-star_st, pl-hsa-miR-720_st, pl-hsa-miR-769-3p_st, pl-hsa-miR-769-5p_st, pl-hsa-miR-874_st, pl-hsa-miR-92a-1-star_st, pl-hsa-miR-941_st, sm-hsa-miR-1263_st, and sm-hsa-miR-202_st.
-18. (canceled)
Dependencies are inferred from the claim text.