US 2015/0031560 A1Published application

Sequencing by orthogonal synthesis

AssigneeIllumina, Inc.

InventorsMartin Maria FABANI, Maria Candelaria ROGERT BACIGALUPO, John A. MOON

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Abstract

A method for sequencing includes steps of (a) providing first and second nucleic acid templates, wherein the two templates have different sequences; (b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs; (c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analog is different from the second set of nucleotide analog, (d) detecting the first and second primer extension products; and (e) repeating steps (b) through (d), thereby determining the different sequences of the first and second templates.

Claims30

Dependent claims

A method for sequencing nucleic acid templates, comprising

(a) providing an array of sites, wherein each site comprises a first nucleic acid template and a second nucleic acid template,
wherein the first nucleic acid template has a sequence that is different from the sequence of the second nucleic acid template;
(b) extending a first primer bound to the first template using a first polymerase species and a first set of nucleotide analogs, thereby producing a first primer extension product comprising a first nucleotide analog at each of the sites;
(c) extending a second primer bound to the second template using a second polymerase species and a second set of nucleotide analogs, thereby producing a second primer extension product comprising a second nucleotide analog at each of the sites,
wherein the first polymerase species is different from the second polymerase species and wherein the first set of nucleotide analogs is different from the second set of nucleotide analogs;
(d) detecting the first primer extension product and the second primer extension product at each of the sites;
and
(e) repeating steps (b) through (d), thereby determining the different sequences of the first template and the second template at each of the sites.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 14/314,864Filed 25 Jun 2014
Priority: 3 Jul 2013
Published: 29 Jan 2015
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12Q 1/6869
Involving nucleic acids: methods for sequencing
C07H 21/04
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of…: with…
C12Q 1/6874
Methods for sequencing: involving nucleic acid arrays, e.g. sequencing by hybridisation
C07H 21/02
Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of…: with…