Pesticidal flavobacterium strain and bioactive compositions metabolites and uses

An isolated strain of a Flavobacterium sp. that has the following characteristics:

• (A) a 16S rRNA gene sequence comprising the forward sequence having at least 99% identity to the sequence set forth in SEQ ID NO:3 and a reverse sequence having at least 99% identity to the sequence set forth in SEQ ID NO:4 and a consensus sequence having at least 99% identity to the sequence set forth in SEQ ID NO:5;
• (B) pesticidal activity;
• (C) growth modulating activity;
• (D) produces a pesticidal compound having the following properties:
• (1) has a molecular weight of about 150-195 as determined by Liquid Chromatography/Mass Spectroscopy (LC/MS);
• (2) has 1H NMR values of δ 7.60, 7.52, 6.83, 6.68, 2.74, 1.14 and has 13C NMR values of 203.96, 161.90, 145.11, 131.78, 131.78, 127.28, 123.83, 117.24, 117.24, 34.52, 8.89;
• (3) has an High Pressure Liquid Chromatography (HPLC) retention time of about 8-14 minutes, on a reversed phase C-18 HPLC (Phenomenex, Luna 5μ C18(2) 100 A, 100×4.60 mm) column using a water:acetonitrile (CH3CN) with a gradient solvent system (0-20 min;
• 90-0% aqueous CH3CN, 20-24 min;
• 100% CH3CN, 24-27 min;
• 0-90% aqueous CH3CN, 27-30 min;
• 90% aqueous CH3CN) at 0.5 mL/min flow rate and UV detection of 210 nm.;
• (E) is non-pathogenic to vertebrate animals;
• (F) is susceptible to tetracycline, erythromycin, streptomycin, penicillin, ampicillin, oxytetracycline, chloramphenicol, ciprofloxacin, gentamicin, piperacillin, imipenem and sulphamethoxazole-trimethoprim or substantially pure filtrate, supernatant, or extract derived therefrom.