Tissue-engineered constructs
AssigneeHumacyte, Inc.
InventorsShannon L.M. Dahl, Laura E. Niklason, Justin T. Strader, William E. Tente, Joseph J. Lundquist
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Abstract
The present invention provides constructs including a tubular biodegradable polyglycolic acid scaffold, wherein the scaffold may be coated with extracellular matrix proteins and substantially acellular. The constructs can be utilized as an arteriovenous graft, a coronary graft, a peripheral artery bypass conduit, or a urinary conduit. The present invention also provides methods of producing such constructs.
Claims13
A construct comprising a tubular biodegradable non-woven polyglycolic acid scaffold comprising non-biodegradable polyethylene terephthalate supports at each end of the tubular biodegradable polyglycolic acid scaffold, wherein the density of the polyglycolic acid is about 45 mg/cc to about 75 mg/cc and said density is uniform across the entire tubular scaffold, wherein the thickness of the polyglycolic acid is about 0.8 to about 1.2 mm, wherein the thickness of the fibers within the polyglycolic acid is about 5 to about 20 μm, and wherein the porosity of the polyethylene terephthalate is >200 cc/min/cm2 and the supports permit the attachment and growth of cells.
A method of producing a tubular polyglycolic acid construct comprising:
- (a) providing a biodegradable polyglycolic acid sheet, wherein the density of the polyglycolic acid is about 45 mg/cc to about 75 mg/cc and the thickness of the polyglycolic acid sheet is about 0.8 to about 1.2 mm,
- (b) wrapping the polyglycolic acid sheet around a mandrel such that opposite edges of the polyglycolic acid sheet meet at an interface;
- (c) pulling polyglycolic acid fibers from each opposing edge of the sheet across the interface, and
- (d) forming a seam by entangling said pulled polyglycolic acid fibers from one side of the interface with the polyglycolic acid fibers on the opposite side of the interface, wherein the density of the polyglycolic acid at the seam is about 45 mg/cc to about 75 mg/cc and the thickness of the polyglycolic acid at the seam is about 0.8 to about 1.5 mm,
- thereby producing a tubular biodegradable polyglycolic acid construct with a uniform polyglycolic acid density.
Dependencies are inferred from the claim text.