Culture medium for eukaryotic cells

A process of producing a culture medium for culturing eukaryotic cells comprising adding to conventional culture medium the following ingredients:

• (i) one or more C2-C6 alpha-hydroxy acids, salts of these acids, esters of these acids and combinations thereof;
• and
• (ii) one or more compounds selected from the following groups of compounds:
• a) amino acid derivatives consisting of γ-glutamyl amino acids, pyroglutamyl amino acids, glutamate-containing or proline-containing dipeptides, oxo-aminoacids, homo-amino acids, N-acetyl amino-acids and glycyl-glycine;
• b) phenolic acid derivatives;
• c) linear C2-C6 or cyclic C6 sugar alcohols;
• d) pyridinic acid derivatives;
• and
• e) nucleobases and/or nucleotides chosen from uracil, adenine, adenosine 3′-monophosphate (3′-AMP) and adenosine 5′-monophosphate (AMP), or a combination of compounds selected from compound groups a), b), c), d) and e),
• as a growth-promoting and/or production-improving ingredient,
• such that the final concentration in the culture medium is at least 0.001 mg/l per individual compound listed under (i) and (ii), wherein said compounds are added as a pure substance or as a concentrate.