US 2015/0031121 A1 · SAMPLE HOLDING CARRIER AND FLUORESCENCE DETECTION DEVICE USING SAME

Abstract

Disclosed is a sample holding carrier allowing samples to be measured accurately, and a fluorescence detection device for use with the sample holding carrier. A biosensor substrate includes a base substrate, a plurality of wells formed on a first surface side of the base substrate; and grooves formed on the first surface side of the base substrate separately from the wells and generating fluorescence under exposure to excitation light. The fluorescence detection device applies excitation light to the grooves, thereby figuring out the level of the fluorescence to be detected from the biosensor substrate. As a result, the fluorescence detection device can amplify the detection signals of the fluorescence generated when the excitation light is applied to the wells to an appropriate level, thereby accurately detecting the fluorescence generated in the samples.

Claims12

Dependent claims

A sample holding carrier comprising:

a substrate;
a plurality of sample accommodation units arranged on a first surface side of the substrate;
and
a fluorescent portion arranged on the first surface side of the substrate separately from the sample accommodation units and generating fluorescence under exposure to irradiation light.

1 more element

A fluorescence detection device that irradiates a sample holding carrier holding a fluorescently-labeled sample with irradiation light and detects fluorescence yielded from the sample irradiated with the irradiation light,

wherein the sample holding carrier comprises:
a substrate;
a plurality of sample accommodation units arranged on a first surface side of the substrate;
and
a fluorescent portion arranged on the first surface side of the substrate separately from the sample accommodation units and generating fluorescence under exposure to the irradiation light;
the fluorescence detection device comprising:
a light source for emitting the irradiation light;
an object lens for converging the irradiation light on the sample holding carrier;
a fluorescence detector for receiving the fluorescence generated from the samples or from the fluorescent portion by exposure to the irradiation light;
and
an amplification rate setting unit for setting amplification rate of a signal sent from the fluorescence detector based on the signal sent from the fluorescence detector when the fluorescent portion is exposed to the irradiation light.

8 more elements

Dependencies are inferred from the claim text.

Sample holding carrier and fluorescence detection device using same

Life of this patent

Sample holding carrier and fluorescence detection device using same

Life of this patent

Abstract

Claims12