Polyol oxidases
AssigneeDanisco US Inc.
InventorsManoj KUMAR, Susan M. MADRID, Hugh C. MCDONALD, Ayrookaran J. POULOSE, Thomas RAND, Huaming WANG
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Abstract
The present invention provides compositions and methods for producing a polyol oxidase in micoroorganisms, and the use of polyol oxidases in cleaning compositions. The invention includes cleaning compositions that contain combinations of two or more POx oxidases, and cleaning compositions that contain combinations of two or more POx oxidases and a perhydrolase. In particular, the invention provides methods for expressing polyol oxidases in bacterial hosts for use in detergent applications for cleaning, bleaching and disinfecting.
Claims19
An isolated chimeric polynucleotide comprising a sequence encoding a mature polyol oxidase enzyme (POx) comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:4, said sequence being operably linked to a sequence encoding a heterologous secretory signal peptide.
(canceled)
A host cell comprising a chimeric polynucleotide comprising a sequence encoding a mature polyol oxidase enzyme (POx) comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:4, said sequence being operably linked to a sequence encoding a heterologous secretory signal peptide.
A method for producing a polypeptide having POx activity comprising:
- (a) transforming a host cell with a recombinant expression vector, said expression vector comprising a polynucleotide sequence encoding a POx polypeptide comprising an amino acid sequence that is at least 90% identical to SEQ ID NO:4;
- (b) growing said host cell comprising said recombinant expression vector under conditions suitable for the expression of said POx polypeptide;
- and
- (c) recovering said POx polypeptide.
16-28. (canceled)
Dependencies are inferred from the claim text.