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US 2015/0031107 A1Published application
Pcr reaction mixtures with decreased non-specific activity
AssigneeBio-Rad Laboratories, Inc.
InventorMAN CHENG
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Abstract
The present invention provides methods for improving the specificity of nucleic acid amplification comprising incubating a nucleic acid molecule with a polymerase-Sso7 DNA binding domain conjugate and arginine, spermidine, or spermine. The present invention also provides reaction mixtures and kits for improving the specificity of nucleic acid amplification.
Claims10
A reaction mixture comprising:
- a polymerase;
- and
- from about 1 mM to about 25 mM of free arginine, or a salt thereof.
Dependencies are inferred from the claim text.