Bacterial cell having enhanced succinic acid production and a method for producing the succinic acid using the same
AssigneeSamsung Electronics Co., Ltd.
InventorsJoonsong PARK, Jinhwan Park, Soonchun Chung, Jiae Yun, Kwangmyung Cho
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Abstract
A genetically engineered bacterial cell wherein activity of a pathway in the cell of converting α-ketoglutarate into succinate semialdehyde; or activity of succinyl semialdehyde dehydrogenase in the cell is increased compared to the activity in a non-genetically engineered cell of the same type, and a method of producing succinic acid by using the same.
Claims22
A genetically engineered bacterial cell, wherein activity of a pathway in the cell of converting α-ketoglutarate (AKG) into succinate semialdehyde (SSA) or activity of succinyl semialdehyde dehydrogenase (SSADH) in the cell is increased as compared to the activity in a parent cell, wherein the pathway of converting AKG into SSA includes α-ketoglutarate decarboxylase (KGDC);
- glutamate:succinate semialdehyde transaminase and glutamate decarboxylase;
- or a combination thereof, and the KGDC catalyzes the conversion of α-ketoglutarate into SSA, and the SSADH catalyzes the conversion of SSA into succinic acid.
A method of improving succinic acid production by a bacterial cell, the method comprising increasing the activity of succinyl semialdehyde dehydrogenase (SSADH), α-ketoglutarate decarboxylase (KGDC), glutamate:succinate semialdehyde transaminase, and glutamate decarboxylase, or a combination thereof, in the cell.
Dependencies are inferred from the claim text.