Enone reductases

A method of reducing an α,β unsaturated compound, the method comprising:

• (a) providing:
• (i) an α,β unsaturated compound selected from the group consisting of an α,β unsaturated ketone, an α,β unsaturated aldehyde, an α,β unsaturated nitrile, and an α,β unsaturated ester;
• and (ii) an enone reductase polypeptide comprising an amino acid sequence that is at least 80% identical to a SEQ ID NO:
• 8, wherein the amino acid sequence comprises one or more residue differences as compared to SEQ ID NO:8, selected from:
• the residue corresponding to X5 is E;
• residue corresponding to X10 is P;
• residue corresponding to X28 is P;
• residue corresponding to X38 is N or S;
• residue corresponding to X40 is L, Y, E or S;
• residue corresponding to X44 is Y;
• residue corresponding to X75 is L or S;
• residue corresponding to X83 is L, R, V, I, K, E, or M;
• residue corresponding to X117 is C, L, A, M, V, I, N, Q, E, F, or S;
• residue corresponding to X119 is P or V;
• residue corresponding to X122 is T;
• residue corresponding to X124 is G or P;
• residue corresponding to X147 is G;
• residue corresponding to X148 is R;
• residue corresponding to X153 is E;
• residue corresponding to X154 is R;
• residue corresponding to X179 is R;
• residue corresponding to X209 is D;
• residue corresponding to X240 is R;
• residue corresponding to X248 is C;
• residue corresponding to X251 is A;
• C, R, E, D, W, Y, R, S, V, G, L, or I;
• residue corresponding to X252 is H;
• residue corresponding to X255 is P;
• residue corresponding to X259 is G;
• residue corresponding to X294 is A;
• residue corresponding to X295 is T, N, or G;
• residue corresponding to X296 is G, F, A, S, R, E, Q, K, or I;
• residue corresponding to X297 is F;
• K;
• Y, W, G, A, or I;
• residue corresponding to X302 is G;
• residue corresponding to X304 is K;
• residue corresponding to X305 is S;
• residue corresponding to X311 is E;
• residue corresponding to X315 is P;
• residue corresponding to X330 is Y or R;
• residue corresponding to X333 is A;
• residue corresponding to X339 is Q;
• residue corresponding to X358 is A;
• residue corresponding to X369 is E;
• residue corresponding to X376 is T, K, I, A, or E;
• residue corresponding to X379 is G;
• residue corresponding to X384 is I;
• residue corresponding to X386 is D;
• residue corresponding to X397 is R;
• residue corresponding to X399 is E;
• and residue corresponding to X400 is T;
• and
• (b) contacting the α,β unsaturated compound with the enone reductase polypeptide under reaction conditions suitable for conversion of the α,β unsaturated compound to the corresponding saturated product compound selected from the group consisting of a saturated ketone, a saturated aldehyde, a saturated nitrile, and a saturated ester.