Polypeptides having alpha-amylase activity and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsMing Li, Junxin Duan, Noriko Tsutsumi, Guillermo Coward-Kelly, Henrik Lundkvist
Life of this patent
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Abstract
The present invention provides isolated polypeptides having alpha-amylase activity catalytic domains, carbohydrate binding domains and polynucleotide encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also provides nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains.
Claims15
An isolated polypeptide having alpha-amylase activity, selected from the group consisting of:
- (a) a polypeptide having at least 80%, e.g., at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO:
- 4;
- or a polypeptide having at least 65%, e.g., at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions, medium stringency conditions, medium-high stringency conditions, high stringency conditions, or very high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 3, or the mature polypeptide coding sequence of SEQ ID NO:
- 1, (ii) the cDNA sequences thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 80%, e.g., at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 3 or the cDNA sequence thereof;
- or a polypeptide encoded by a polynucleotide having at least 65%, e.g., at least 70%, at least 75%, at least 80%, at least 85%, at least 90%, at least 91%, at least 92%, at least 93%, at least 94%, at least 95%, at least 96%, at least 97%, at least 98%, at least 99%, or 100% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 4 or the mature polypeptide of SEQ ID NO:
- 2 comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has alpha-amylase activity.
An isolated polypeptide comprising a catalytic domain selected from the group consisting of:
- (a) a catalytic domain having at least 80% sequence identity to amino acids 21 to 493 of SEQ ID NO:
- 4, or at least 65% sequence identity to amino acids 26 to 503 of SEQ ID NO:
- 2;
- (b) a catalytic domain encoded by a polynucleotide that hybridizes under low, medium, medium-high, high, or very high stringency conditions with nucleotides 61 to 1973 of SEQ ID NO:
- 3, or nucleotides 76 to 1578 of SEQ ID NO:
- 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a catalytic domain encoded by a polynucleotide having at least 80% sequence identity to nucleotides 61 to 1973 of SEQ ID NO:
- 3, or at least 65% sequence identity to nucleotides 76 to 1578 of SEQ ID NO:
- 1;
- (d) a variant of amino acids 21 to 493 of SEQ ID NO:
- 4 or amino acids 26 to 503 of SEQ ID NO:
- 2, comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the catalytic domain of (a), (b), (c), or (d) that has alpha-amylase activity.
An isolated polypeptide comprising a carbohydrate binding domain, wherein the binding domain is selected from the group consisting of:
- (a) a carbohydrate binding domain having at least 80% sequence identity to amino acids 511 to 619 of SEQ ID NO:
- 4;
- (b) a carbohydrate binding domain encoded by a polynucleotide that hybridizes under low, medium, medium-high, high, or very high stringency conditions with (i) nucleotides 2025 to 2351 of SEQ ID NO:
- 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a carbohydrate binding domain encoded by a polynucleotide having at least 80% sequence identity to nucleotides 2025 to 2351 of SEQ ID NO:
- 3 or the cDNA sequence thereof;
- (d) a variant of amino acids 511 to 619 of SEQ ID NO:
- 4 comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of (a), (b), (c), (d) or (e) that has carbohydrate binding activity.
An isolated polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 20 of SEQ ID NO:
- 4, or amino acids 1 to 25 of SEQ ID NO:
- 2.
(canceled)
Dependencies are inferred from the claim text.