Gene synthesis process, gene chip and kit
AssigneeJingdong Tian
InventorJingdong Tian
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Abstract
Provided is a chip process of gene synthesis, and the process comprises incorporating the whole procedure, which comprises amplifying oligonucleotides and assembling the oligonucleotides into a gene in parallel, onto a single chip. A specific mismatch endonuclease is also used in the process to establish an error repair system in gene synthesis, and the error rate is decreased to about 0.19 mismatched bases/kb. The high-throughput, high-fidelity and low-cost chip process of gene synthesis provided in the present invention can meet the requirements of gene synthesis and the optimization and screening of protein expression on a large scale at the frontier of life sciences such as synthetic biology, genomics, and systems biology.
Claims13
A method of gene synthesis, characterized in that oligonucleotide amplification and gene assembly are performed on one gene chip by using isothermal nicking and strand displacement amplification and polymerase cycling assembly reactions;
- the gene chip is formed by immobilizing oligonucleotides to the surface of a solid substrate, the oligonucleotide has at the 3′-end a linker sequence of 15-150 bases in length and is anchored onto the surface of the chip via a nicking endonuclease recognition site within the linker sequence.
A gene chip formed by immobilizing oligonucleotide probes onto the surface of a solid substrate, characterized in that the oligonucleotide has at the 3′-end a linker sequence of 15-150 bases in length and is anchored onto the surface of the chip via a nicking endonuclease recognition site within the linker sequence.
Dependencies are inferred from the claim text.