US 2015/0031085 A1 · Novel Leech Hyaluronidase and Its Application

Abstract

The present invention provides a novel leech HAase and a method of producing low-molecular-weight HA oligosaccharides using the leech HAase. This invention successfully cloned the first leech HAase gene and provides a method for high-level expression of the leech HAase gene. By controlling the incubation condition, different HA oligosaccharides, particularly HA4, HA6, HA8 and HA10, can be selectively generated using the leech HAase. The large-scale expression of the leech HAase and the enzymatic production of specific HA oligosaccharides are not only useful for the cosmetic, healthcare and the medical industries but also can be a great help to polysaccharides chemical synthesis and cancer research.

Claims12

Dependent claims

An isolated leech hyaluronidase (HAase), wherein the nucleotide sequence encoding said HAase comprises a member selected from the group consisting of:

a) SEQ ID NO:1;
b) a nucleotide sequence with one or several nucleotides substituted, deleted or added based on SEQ ID NO:1;
c) a nucleotide sequence having 85% identity with SEQ ID NO:1;
d) a nucleotide sequence encoding a polypeptide of SEQ ID NO:3;
e) a nucleotide sequence with one or several nucleotides substituted, deleted, or added based on a nucleotide sequence of d);
and
f) a nucleotide sequence having 85% identity with a sequence of d).

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/251,579Filed 12 Apr 2014

Priority

CN 201310323064.029 Jul 2013

Published

29 Jan 2015

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C12N 9/2402
Acting on glycosyl compounds: hydrolysing O- and S- glycosyl compounds
C12P 19/26
Preparation of compounds containing saccharide radicals: preparation of nitrogen-containing carbohydrates
C12Y 302/01036
Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds: hyaluronoglucuronidase
C12P 21/02
Preparation of peptides or proteins: having a known sequence of two or more amino acids, e.g. glutathione

Novel Leech Hyaluronidase and Its Application

Life of this patent

Novel Leech Hyaluronidase and Its Application

Life of this patent

Abstract

Claims12