Methods and materials for reducing degradation of recombinant proteins
AssigneeOxyrane UK Limited
InventorsWouter Vervecken, Stefan Ryckaert
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Abstract
Described herein are methods and materials for reducing degradation of recombinant proteins in fungal cells such as Yarrowia.
Claims27
An isolated Yarrowia cell genetically engineered to comprise a deficiency in pYPS1 (YPS1 protein) activity and a deficiency in pYPS2 (YPS2 protein) activity.
A substantially pure culture of Yarrowia lipolytica cells, a substantial number of which are genetically engineered to comprise a deficiency in pYPS1 activity and a deficiency in pYPS2 activity.
An isolated Yarrowia cell genetically engineered to comprise (i) a deficiency in pYPS1 activity and (ii) a deficiency in pYPS2 activity, and one or more of (iii) a deficiency in ALG3 activity, (iv) a deficiency in OCH1 activity, (v) a nucleic acid encoding an alpha-1,2 mannosidase, (vi) a nucleic acid encoding a GlcNAc-transferase I, (vii) a nucleic acid encoding a GlcNAc-transferase II, (viii) a nucleic acid encoding a mannosidase II, (ix) a nucleic acid encoding an α-1,3-glucosyltransferase, (x) a nucleic acid encoding a galactosyltransferase, and (xi) a nucleic acid encoding the α and β subunits of a glucosidase.
A method for producing a target protein, said method comprising a) providing a Yarrowia cell genetically engineered to comprise a deficiency in pYPS1 activity, a deficiency in pYPS2 activity, and a nucleic acid encoding said target protein;
- and b) culturing said cell under conditions such that said cell produces said target protein.
Dependencies are inferred from the claim text.