Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorNikolaj Spodsberg
Life of this patent
Estimating the term…
Abstract
The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims19
An isolated polypeptide having xylanase activity, selected from the group consisting of:
- (a) a polypeptide having at least 77% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, or a polypeptide having at least 77% sequence identity to the mature polypeptide of SEQ ID NO:
- 4;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- or the cDNA sequence thereof;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 2 or SEQ ID NO:
- 4 comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has xylanase activity.
An isolated polypeptide comprising a catalytic domain selected from the group consisting of:
- (a) a catalytic domain having at least 77% sequence identity to the catalytic domain of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- (b) a catalytic domain encoded by a polynucleotide having at least 60% sequence identity to the catalytic domain coding sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- (c) a variant of a catalytic domain comprising a substitution, deletion, and/or insertion of one or more (several) amino acids of the catalytic domain of SEQ ID NO:
- 2 or SEQ ID NO:
- 4;
- and
- (d) a fragment of a catalytic domain of (a), (b), or (c), which has xylanase activity.
A recombinant host cell transformed with a nucleic acid construct or expression vector comprising a polynucleotide operably linked to one or more control sequences that direct the production of a polypeptide in the recombinant host cell, wherein the polypeptide is selected from the group consisting of:
- (a) a polypeptide having at least 77% sequence identity to the mature polypeptide of SEQ ID NO:
- 2, or a polypeptide having at least 77% sequence identity to the mature polypeptide of SEQ ID NO:
- 4;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under low stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or SEQ ID NO:
- 3;
- or the cDNA sequence thereof;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 2 or SEQ ID NO:
- 4 comprising a substitution, deletion, and/or insertion at one or more positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has xylanase activity.
Dependencies are inferred from the claim text.