Bioanalytical Reagent used in Heterogeneous Phase and Usage Method Thereof

A bioanalytical reagent, including a target detector and a signal generator, the said target detector connect the signal generator via three ways, (1) directly connected;

• (2) indirect coupling through a linker, spacer or adapter;
• (3) indirect coupling through carrier, wherein, the signal generator comprises the following formula:
• (p1)y-(a trigger)z-(LS)N-a degradable polymer coupled with contrast agent in latent state via covalent bonds-p2
• Wherein,
• (1) Z:
• the number of the trigger, is a non-negative integer, Z trigger are the same or different, but only when the first trigger closed to the p1 is stimulated, the second trigger of the p1 end can be stimulated, and then the third, and so on;
• (2) p1 is a protecting group for the end group of the trigger, also is the connection portion between the target detector and the signal generator, y:
• the number of p1, its value is 0 or 1;
• (3) (LS)N is self-immolative linker or spacer, the N LS are the same or different, N is a non-negative integer;
• (4) p2 is a protecting group for the end group of the degradable polymer, also is the connection portion between the target detector and the signal generator;
• the said signal generator is in a latent state during wash and separation procedures (contrast reagents issue a weak signal or no signal under the excitation of the external signal source), after the separation procedure, add or apply inducing matter or a condition which excites the contrast agent from a latent state to an active state into the analysis system to degrade directly the polymer or to stimulate the triggers in sequence before degrade the polymer (use the external signal when it is necessary), so, the detection signal is excited or its intensity is remarkably increased.