Method of identifying vdj recombination products

A method of identifying a VDJ recombination product which comprises the following steps:

• (a) obtaining a nucleic acid sample comprising a VDJ recombination product;
• (b) when said nucleic acid sample comprises DNA, fragmenting the VDJ recombination product either by sonication, shearing or performing a restriction endonuclease reaction at a first site downstream of each of the J genes or downstream of the constant region, and a second site within or immediately upstream of the V gene to generate digested VDJ recombined fragments and unrecombined J fragments;
• (c) when said nucleic acid sample comprises DNA, annealing oligonucleotides to the digested fragments at unique regions within or immediately downstream of each of the J genes, and when the nucleic acid sample is cDNA or RNA, annealing oligonucleotides to the VDJ recombination product at a position specific to the constant region or J gene;
• (d) when said nucleic acid sample comprises DNA, separating the digested VDJ recombined fragments from the unrecombined J fragments and the rest of the genome;
• (e) sequencing the VDJ recombined fragments or products;
• and
• (f) data processing of the sequencing data to identify each VDJ recombination product and quantify VDJ recombination frequencies.