Method of detecting residual genomic dna and a kit thereof
AssigneeBiocon Limited
InventorsRahul Sharad Fadnis, Reena Nichinmetia Raghunandan
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Abstract
The present disclosure relates to a highly specific and sensitive method of detecting host cell impurities in a biological sample by using quantitative real time polymerase chain reaction (q PCR). The present disclosure also provides novel designed primer and probe to amplify only the specific Alu family of dispersed repetitive sequences from Chinese hamster ovary cells used for expression of therapeutic proteins.
Claims23
A sensitive and quantitative method to detect the residual genomic DNA from a host cell of rodent family as low as 20 femtogram in a protein product by using primer sequence ID number 22 and sequence ID number 23 and corresponding probe sequence ID number 24 targeted to amplify Alu family of the dispersed sequences.
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