Method of dna detection and quantification by single-molecule hybridization and manipulation
AssigneesCentre National De La Recherche Scientifique, Ecole Normale Superieure, Universite Pierre Et Marie Curie (Paris)
InventorsDavid Bensimon, Jean-Francois Allemand, Fang-Yuan Ding, Vincent Croquette
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Abstract
The present invention relates to a fast method for the detection and the quantification of a nucleic acid, DNA or RNA. Specifically, the invention provides a method for detecting and quantifying the presence of a specific nucleic acid molecule which is based on physical and electronic treatments. The method of the invention is particularly useful for applications as diverse as detection of chromosomal abnormal distributions or gene expression analysis.
Claims21
A method for quantifying a species of double-stranded nucleic acid molecules comprising a specific nucleic acid sequence in a sample, said method comprising:
- a) denaturing said double-stranded nucleic acid molecules in the sample by applying a physical force to said molecules;
- b) providing a single-stranded nucleic acid molecule corresponding to said sequence;
- c) renaturing said denatured double-stranded nucleic acid molecules of a) in the presence of said single-stranded nucleic acid molecule;
- d) detecting the double-stranded nucleic acid molecules where renaturation is blocked;
- and
- e) numerating the double-stranded nucleic acid molecules of d).
A method for quantifying a species of double-stranded nucleic acid molecules comprising one or more specific sequence in a sample, said method comprising:
- a) denaturing said double-stranded nucleic acid molecules in the sample by applying a physical force to said molecules;
- b) providing one or more single-stranded nucleic acid molecule corresponding to said one or more sequences;
- c) renaturing said denatured double-stranded nucleic acid molecules of a) in the presence of said single-stranded nucleic acid molecule;
- and
- d) detecting the double-stranded nucleic acid molecules where renaturation is blocked by said one or more single-stranded nucleic acid molecules;
- and
- e) numerating the double-stranded nucleic acid molecules of d).
Dependencies are inferred from the claim text.