USE OF SAA1 beta/beta HOMOZYGOTE IN THE PROGNOSIS DIAGNOSIS AND DIAGNOSIS OF LIVER CIRRHOSIS
AssigneeDiasys Diagnostic Systems (Shanghai) Co., Ltd.
InventorsRongfang Wang, Jun Wu, Yan Zhang, Zhenbin Qian
Life of this patent
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Abstract
Provided is uses of SAA1β/β(1.5/1.5) homozygote in prognosis and/diagnosis of liver cirrhosis, and in preparation of a reagent for liver cirrhosis prognosis and/or liver cirrhosis diagnosis. Recognition of liver cirrhosis susceptible populations can be achieved by detection of human SAA1β/β homozygote and non-SAA1β/β homozygote through real-time fluorescence quantitative allele specific PCR.
Claims19
Use of SAA1β/β homozygote in diagnosis of liver cirrhosis and/or in prognosis of hepatitis B associated liver cirrhosis.
Use of SAA1β/β homozygote in the preparation of a reagent for liver cirrhosis prognosis and/or diagnosis.
Use of SAA1β/β homozygote as a liver cirrhosis biomarker in the diagnosis of liver cirrhosis.
Use of SAA1β/β homozygote as a risk factor in the prognosis of liver cirrhosis.
A method of identifying liver cirrhosis susceptible populations by SAA1 genotyping, comprising the steps of constructing synthetic primers according to the gene sequences of three SAA1α, β and γ alleles, amplifying samples to be tested by real-time allele-specific PCR, and determining the genotypes of the PCR-amplified products based on CT value and melting curve;
- when the PCR-amplified product is determined as SAA1β/β homozygote, the sample is then determined as a susceptible population to liver cirrhosis.
A diagnosis reagent for liver cirrhosis prognosis and/or diagnosis, comprising the primers, the genomic DNA of human SAA1α, β, γ alleles to be tested, a pfu DNA polymerase, SYBR GREEN I fluorescent dye and ROX fluorescence calibration solution;
- the primers comprise:
- 1) A pair of primers of group A3:
- Forward primer 5′-3′ sequence (SEQ ID NO:
- 1):
- TCCCTTCTGCCTTTCCTTTCCTTTCC,
- Reverse primer 5′-3′ sequence (SEQ ID NO:
- 2):
- TTACGTGATCGCTTCTGCAGCCCAGG;
- 2) A pair of primers of group A2:
- Forward primer 5′-3′ sequence (SEQ ID NO:
- 1):
- TCCCTTCTGCCTTTCCTTTCCTTTCC,
- Reverse primer 5′-3′ sequence (SEQ ID NO:
- 3):
- TTACGTGATCGCTTCTGCAGCCCAGA;
- 3) A pair of primers of group B3:
- Forward primer 5′-3′ sequence (SEQ ID NO:
- 1):
- TCCCTTCTGCCTTTCCTTTCCTTTCC,
- Reverse primer 5′-3′ sequence (SEQ ID NO:
- 4):
- TCCCAGGAGCTCCAGTTACGTGATCG;
- 4) A pair of primers of group B2:
- Forward primer 5′-3′ sequence (SEQ ID NO:
- 1):
- TCCCTTCTGCCTTTCCTTTCCTTTCC.
Dependencies are inferred from the claim text.