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US 2015/0031023 A1Published application
Method of detecting nucleic acids containing a genetic variation
AssigneeSamsung Electronics Co., Ltd.
InventorsDong-hyun PARK, Yeon-jeong Kim, Kyung-yeon Han
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Abstract
Provided are a composition or a kit for detecting a nucleic acid with genetic variation including a first amplification blocking nucleic acid and a second amplification blocking nucleic acid, and a method of detecting a nucleic acid with genetic variation by using the same. Based on the above, a nucleic acid with genetic variation can be detected with high sensitivity and accuracy.
Claims20
A method of detecting a nucleic acid with genetic variation, the method comprising:
- (a) incubating a sample including a target nucleic acid comprising a region of a wild type gene known to exhibit genetic variation with
- a primer pair;
- a first amplification blocking nucleic acid comprising a sequence that is the same as or complementary to at least two consecutive nucleotides of the region of the wild type gene known to exhibit genetic variation;
- and
- a second amplification blocking nucleic acid comprising a sequence complementary to at least two consecutive nucleotides of the first amplification blocking nucleic acid;
- (b) amplifying the target nucleic acid with a polymerase, thereby producing an amplification product;
- and
- (c) detecting the amplification product to detect a nucleic acid with genetic variation.
5 more elements
A method of detecting a nucleic acid with genetic variation, the method comprising:
- (a) incubating a sample including a target nucleic acid comprising a region of a wild type gene known to exhibit genetic variation with
- a first primer pair and
- a first amplification blocking nucleic acid,
- wherein the first amplification blocking nucleic acid comprises a sequence that is the same as or complementary to at least two consecutive nucleotides of the region of the wild type gene known to exhibit genetic variation;
- (b) amplifying the target nucleic acid with a nucleic acid polymerase to provide a first amplification product;
- (c) incubating the first amplification product with
- a second primer pair and
- a second amplification blocking nucleic acid,
- wherein the second amplification blocking nucleic acid comprises a sequence complementary to at least two consecutive nucleotides of the first amplification blocking nucleic acid;
- (d) amplifying the first amplification product with a nucleic acid polymerase to provide a second amplification product;
- and
- (e) detecting the second amplification product to detect a nucleic acid with genetic variation.
9 more elements
Dependencies are inferred from the claim text.