Methods For Diagnosing Feline Coronavirus Infections
OwnerGary Whittaker, Andrew Reganinventors
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Abstract
Provided is a method for determining whether a feline is infected with pathogenic Feline Infectious Peritonitis Virus (FIPV) or Feline Enteric Infection Virus (FECV). The method involves determining the presence or absence of intact or mutated S1/S2 and S2′ cleavage sites in the spike protein of serotype 1 feline coronaviruses (FCoV1). The presence of both intact cleavage sites is indicative of FECV. The presence of a mutation in one or both cleavage sites is indicative of FIPV. The absence of both sites is indicative of an absence of FCoV1 infection. Compositions for use in determining infection and kits are also provided.
Claims9
A composition for determining whether a feline is infected with pathogenic Feline Infectious Peritonitis Virus (FIPV) or Feline Enteric Infection Virus (FECV) comprising:
- a) a polynucleotide sequence for determining the presence or absence of a mutation in SEQ ID NO:5 (Arg Arg Ser Arg Arg Ser) in a type 1 feline coronavirus (FCoV1) spike protein;
- and
- b) a polynucleotide sequence for determining the presence or absence of a mutation of SEQ ID NO:8 (Xaa Xaa Ser Ala Val Glu Asp) in the type 1 FCoV1 spike protein, wherein Xaa is a basic amino acid and wherein the mutation is a change to a non-basic amino acid in the first, second or both Xaa in the SEQ ID NO:8.
A kit for determining whether a feline is infected with pathogenic Feline Infectious Peritonitis Virus (FIPV) or Feline Enteric Infection Virus (FECV), in a single container or separate containers, comprising:
- a) a polynucleotide sequence for determining the presence or absence of a mutation in SEQ ID NO:5 (Arg Arg Ser Arg Arg Ser) in a type 1 feline coronavirus (FCoV1) spike protein;
- and
- b) a polynucleotide sequence for determining the presence or absence of a mutation of SEQ ID NO:8 (Xaa Xaa Ser Ala Val Glu Asp) in the type 1 FCoV1 spike protein, wherein Xaa is a basic amino acid and wherein the mutation is a change to a non-basic amino acid in the first, second or both Xaa in the SEQ ID NO:8.
A composition for determining whether a feline is infected with pathogenic Feline Infectious Peritonitis Virus (FIPV) or Feline Enteric Infection Virus (FECV) comprising:
- a) a specific binder that can distinguish a FCoV1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:5 (Arg Arg Ser Arg Arg Ser) from a FCoV1 polypeptide comprising a mutation in the amino acid sequence set forth in SEQ ID NO:5;
- and
- b) a specific binder that can distinguish a FCoV1 polypeptide comprising an amino acid sequence set forth in SEQ ID NO:8 (Xaa Xaa Ser Ala Val Glu Asp) from a FCoV1 polypeptide comprising a mutation in the amino acid residue(s) Xaa and/or Xaa set forth in SEQ ID NO:8.
Dependencies are inferred from the claim text.