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US 2015/0031014 A1Published application
Detecting analytes with a ph meter
AssigneeThe Board of Trustees of the University of Illinois
InventorsYi Lu, Yu Xiang, Zhe Shen, JingJing Zhang
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Abstract
Provided herein are sensors, kits that include such sensors, and methods for making and using such sensors. The sensors permit detection of a broad array of target molecules, such as nucleic acids (e.g., DNA and RNA), proteins, toxins, pathogens, cells, and metals, and can be used in combination with pH meters and pH paper. Thus, this disclosure provides a new methodology that allows pH meters and pH paper to be used for the detection of analytes other than pH.
Claims20
A method for detecting a target, comprising
- contacting a sample with (a) a recognition molecule specific for the target and (b) a solid support comprising glucose oxidase, under conditions sufficient to allow the target in the sample to bind to the recognition molecule and to release the glucose oxidase from the solid support;
- separating the solid support from the released glucose oxidase;
- contacting the released glucose oxidase with glucose, thereby generating gluconic acid;
- and
- detecting a change in pH, wherein detection of a significant decrease in pH indicates the presence of the target agent in the sample, and an absence of detected significant decrease in pH indicates the absence of the target agent in the sample.
2 more elements
A sensor, comprising
- (a) a solid support comprising
- a first nucleic acid molecule having a 5′-end and a 3′-end, wherein the first nucleic acid is attached to the solid support by the 5′-end, and wherein the first nucleic acid is complementary to a 5′-end of a substrate strand of a DNAzyme specific for a target that can be detected by the sensor;
- and
- a second nucleic acid molecule having a 5′-end and a 3′-end, wherein the 5′-end of the second nucleic acid molecule is hybridized to the 3′-end of the first nucleic acid molecule and wherein the 3′-end of the second nucleic acid molecule comprises glucose oxidase;
- (b) a solid support comprising
- a first nucleic acid molecule having a 5′-end and a 3′-end, wherein the first nucleic acid is attached to the solid support by the 3′-end;
- a second nucleic acid molecule having a 5′-end and a 3′-end, wherein the 3′-end of the second nucleic acid molecule is proximal to the 5′-end of the first nucleic acid molecule and wherein the 5′-end of the second nucleic acid molecule comprises the glucose oxidase;
- and
- an aptamer specific for a target that can be detected by the sensor, wherein the aptamer comprises a nucleic acid molecule having a 5′-end and a 3′-end, wherein the aptamer nucleic acid molecule is complementary and hybridizes to the first nucleic acid molecule and to the second nucleic acid molecule, wherein the 3′-end of the aptamer nucleic acid molecule is not hybridized
- (c) a solid support comprising
- an aptamer nucleic acid molecule having a first end and a second end, wherein the nucleic acid molecule is attached to the solid support by the first end and comprises glucose oxidase on the second end, and wherein the solid support comprises graphene oxide;
- or
- (d) a solid support comprising
- a recognition molecule bound to a target-glucose oxidase complex, wherein in the presence of the target in a sample the amount of target-glucose oxidase complex bound to the solid support decreases, and wherein the amount of target in the sample is proportional to the amount of unbound target-glucose oxidase complexes.
11 more elements
Dependencies are inferred from the claim text.