Generation of Brown Adipose Tissue (BAT) from Mesenchymal Cells
AssigneesNational University of Singapore, Auckland Uniservices Limited
InventorsMichael Raghunath, Michelle Hui Ching Lee, Allan Sheppard
Life of this patent
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Abstract
Methods of generating functional human brown adipocytes, comprising exposing human stem cells, progenitor cells, or white adipocytes to culture with an differentiation cocktail that comprises one or more browning agents (e.g., one or more macromolecular crowders), and optionally one or more adipogenic agents, are described, as are populations of human brown adipocytes generated by the methods, and uses for the populations. Methods of generating functional human brown adipocytes in an individual, such as by administering a pharmaceutical composition comprising an differentiation cocktail, are also described.
Claims26
A method of generating functional human brown adipocytes from human stem cells or progenitor cells, comprising culturing the cells with a differentiation cocktail comprising one or more adipogenic agents and one or more browning agents, wherein the cells thereby differentiate into functional human brown adipocytes.
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The method of claim 4, wherein the macromolecular crowders comprise:
- a) an organic-based hydrophilic macromolecule having a molecular weight of 50 kDa to 500 kDa and a neutral surface charge;
- b) an organic-based hydrophilic macromolecule having a radius range of 2 to 50 nm and a neutral or negative surface charge;
- c) a mixture of two or more of organic-based hydrophilic macromolecules described in (a) and/or (b);
- d) a mixture of at least two types of organic-based hydrophilic macromolecules, each type having a molecular weight of 50 kDa to 500 kDa and a neutral surface charge;
- e) a mixture of at least two types of organic-based hydrophilic macromolecules, each type having a molecular weight of 50 kDa to 500 kDa and neutral surface charge, together with a third type of organic-based hydrophilic macromolecule having a radius range of 2 to 50 nm and a neutral surface charge;
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- f) a mixture of at least two types of organic-based hydrophilic macromolecules, each type having a molecular weight of 50 kDa to 500 kDa and neutral surface charge, together with a third type of organic-based hydrophilic macromolecule having a radius range of 2 to 50 nm and a negative surface charge.
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A method of generating functional human brown adipocytes from human white adipocyte cells, comprising culturing the cells with a differentiation cocktail that comprises one or more browning agents comprising one or more macromolecular crowders, wherein the cells thereby differentiate into functional human brown adipocytes.
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A method of generating functional human brown adipocytes in an individual, comprising administering a pharmaceutical composition comprising a differentiation cocktail to the individual.
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Dependencies are inferred from the claim text.