Activation and Expansion of T Cell Subsets Using Biocompatible Solid Substrates with Tunable Rigidity
AssigneeThe Trustees of Columbia University in the City of New York
InventorsMichael C. Milone, Lance C. Kam, Keyue Shen, Hueli Hao, Roderick O'Connor, Geraldine Goebrecht
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Abstract
The present invention provides compositions and methods for activation and expansion of T cells using a biocompatible solid substrate with tunable rigidity. Rigidity of a substrate is an important parameter that can be used to control the overall expansion and differentiation of T cells.
Claims41
A culture system comprising a biocompatible substrate with tunable rigidity for use in stimulating a T cell, wherein said substrate displays on its surface a first agent that stimulates a TCR/CD3 complex-associated signal in T cells and a second agent that stimulates a CD28 accessory molecule on the surface of the T cells.
A method of stimulating T cells in culture, the method comprising culturing T cells in the presence of a biocompatible substrate with tunable rigidity, wherein said substrate displays on its surface a first agent that stimulates a TCR/CD3 complex-associated signal in T cells and a second agent that stimulates a CD28 accessory molecule on the surface of the T cells.
A culture system comprising a biocompatible substrate with an optimized rigidity for use in stimulating a T cell, wherein said substrate displays on its surface a first agent that stimulates a TCR/CD3 complex-associated signal in T cells and a second agent that stimulates a CD28 accessory molecule on the surface of the T cells.
A method of adoptive immunotherapy comprising obtaining T cells from a subject, stimulating the T cells by culturing the T cells in the presence of a biocompatible substrate with an optimized rigidity, wherein said substrate displays on its surface a first agent that stimulates a TCR/CD3 complex-associated signal in T cells and a second agent that stimulates a CD28 accessory molecule on the surface of the T cells, and administering the stimulated T cells to the subject.
Dependencies are inferred from the claim text.