Pegylated fibrinogen precursor molecule
AssigneeRegentis Biomaterials Ltd.
InventorsDROR SELIKTAR, LIORA ALMANY
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Abstract
The present invention relates to biodegradable scaffolds composed of a naturally-occurring protein backbone cross-linked by a synthetic polymer. Specifically, the present invention provides PEGylated-fibrinogen scaffold and methods of generating and using same for treating disorders requiring tissue regeneration.
Claims19
A composition comprising a precursor molecule which comprises a fibrinogen protein which is denatured and retains an activity of forming a scaffold and at least two PEG molecules covalently connected to free thiol groups of said denatured fibrinogen protein, each of said at least two PEG molecules comprising a functional group for cross-linking, the composition further comprising pegylated fibronectin.
A method of generating a scaffold comprising:
- (a) obtaining a composition of matter comprising plasma-purified fibrinogen;
- (b) generating a plurality of precursor molecules from said composition of matter and PEG which comprise a fibrinogen protein which is denatured and retains an activity of forming a scaffold and at least two PEG molecules covalently connected to free thiol groups of said denatured fibrinogen protein, each of said at least two PEG molecules comprising a functional group for cross-linking;
- and subsequently (c) cross-linking said plurality of precursor molecules to thereby generate the scaffold.
A composition comprising a scaffold which comprises a plurality of fibrinogen molecules and a plurality of synthetic polymers, each of said fibrinogen molecules being covalently attached to at least two of said synthetic polymers, wherein said synthetic polymers are crosslinked and wherein said fibrinogen is denatured using a denaturing agent, the scaffold further comprising pegylated fibronectin.
Dependencies are inferred from the claim text.