Control of phytopathogenic microorganisms with pseudomonas sp. and substances and compositions derived therefrom
AssigneeMarrone Bio Innovations, Inc.
InventorsRatnakar Asolkar, Ana Lucia Cordova-Kreylos, Carly Todd
Life of this patent
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Abstract
Provided are compounds and compositions derived from Pseudomonas sp., particularly, Pseudomonas fluorescens or Pseudomonas protegens and more particularly strain having the identifying characteristics of Pseudomonas ATCC 55799 having antimicrobial properties and particularly, antibacterial properties.
Claims16
An isolated compound having the following characteristics (a) is obtainable from a Pseudomonas species which produces at least one compound that controls zebra, quagga and golden mussels;
- (b) modulates one or more species of phytopathogenic microorganism infestation in a location and (c) has a molecular weight and HPLC retention time selected from the group consisting of:
- (i) a molecular weight of:
- about 300-380 as determined by Liquid Chromatography/Mass Spectroscopy (LC/MS) and an HPLC retention time of about 12-22 minutes, on a reversed phase C-18 HPLC (Phenomenex, Luna 5μ, C18(2) 100 A, 100×4.60 mm) column using a water:
- acetonitrile (CH3CN) with a gradient solvent system (0-20 min;
- 90-0% aqueous CH3CN, 20-24 min;
- 100% CH3CN, 24-27 min;
- 0-90% aqueous CH3CN, 27-30 min;
- 90% aqueous CH3CN) at 0.5 mL/min flow rate and UV detection of 210 nm;
- and has a UV absorption at 212, 252, 312 nm;
- (ii) a molecular weight of about 300-360 as determined by LC/MS;
- and an HPLC retention time of about 10-20 minutes on a reversed phase C-18 HPLC (Phenomenex, Luna 5μ, C18(2) 100 A, 100×4.60 mm) column using a water:acetonitrile (CH3CN) with a gradient solvent system (0-20 min;
- 90-0% aqueous CH3CN, 20-24 min;
- 100% CH3CN, 24-27 min;
- 0-90% aqueous CH3CN, 27-30 min;
- 90% aqueous CH3CN) at 0.5 mL/min flow rate and has a UV absorption at 299, 311, 325 nm;
- (iii) a molecular weight of about 580-680 as determined by LC/MS and an HPLC retention time of 8-20 minutes, on a reversed phase C-18 HPLC (Phenomenex, Luna 5μ, C18(2) 100 A, 100×4.60 mm) column using a water:acetonitrile (CH3CN) with a gradient solvent system (0-20 min;
- 90-0% aqueous CH3CN, 20-24 min;
- 100% CH3CN, 24-27 min;
- 0-90% aqueous CH3CN, 27-30 min;
- 90% aqueous CH3CN) at 0.5 mL/min flow rate and has a UV absorption at 299, 311, 325 nm;
- (iv) a molecular weight of about 350-425 as determined by LC/MS and an HPLC retention time of about 6-16 minutes on a reversed phase C-18 HPLC (Phenomenex, Luna 5μ, C18(2) 100 A, 100×4.60 mm) column using a water:acetonitrile (CH3CN) with a gradient solvent system (0-20 min;
- 90-0% aqueous CH3CN, 20-24 min;
- 100% CH3CN, 24-27 min;
- 0-90% aqueous CH3CN, 27-30 min;
- 90% aqueous CH3CN) at 0.5 mL/min flow rate and UV detection of 210 nm;
- and has a UV absorption at 221, 267, 361 nm.
A method of modulating one or more phytopathogenic micro-organisms in a location where modulation is desired comprising introducing into said location an amount of a cell suspension or whole cell broth comprising cells derived from a Pseudomonas species, wherein said Pseudomonas species produces a one or more substances that control zebra, quagga and golden mussels, or a supernatant, filtrate, cell fraction, one or more metabolites, one or more compounds and/or extract derived therefrom, effective to modulate said phytopathogenic microorganisms.
Use of a cell suspension or whole cell broth comprising cells derived from a Pseudomonas species, wherein said Pseudomonas species produces a one or more substances that control zebra, quagga and golden mussels, or a supernatant, filtrate, cell fraction, one or more metabolites, one or more compounds and/or extract derived therefrom to formulate a composition to modulate phytopathogenic microorganisms.
Dependencies are inferred from the claim text.