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US 2015/0030565 A1Published application
Purification of flaviviruses
AssigneeSanofi Pasteur Biologics, LLC
InventorsSophia Mundle, Stephen Anderson
Life of this patent
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Abstract
The present invention provides a method to prepare purified enveloped (e.g., flavivirus) viral particle preparations employing ion exchange chromatography and tangential flow filtration.
Claims34
A method for the purification of a flavivirus viral particle from a host cell culture comprising the steps of:
- a. recovering flavivirus viral particles from a host cell culture;
- b. subjecting the flavivirus viral particles obtained from step (a) to tangential flow filtration;
- c. applying the retentate from the tangential flow filtration step to an anion exchange chromatography resin;
- d. eluting flavivirus viral particles from the anion exchange chromatography resin;
- and
- e. recovering purified flavivirus viral particles.
3 more elements
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A pharmaceutically acceptable dosage form of flavivirus viral particles produced in a host cell culture, said flavivirus viral particles isolated by a method comprising the steps of:
- a. recovering flavivirus viral particles from a host cell culture;
- b. subjecting the flavivirus viral particles solution obtained from step (a) to tangential flow filtration;
- c. applying the retentate from the tangential flow filtration step to an anion exchange chromatography resin;
- d. eluting flavivirus viral particles from the anion exchange column;
- e. recovering purified flavivirus viral particles;
- and
- f. suspending purified flavivirus viral particles in a pharmaceutically acceptable carrier.
4 more elements
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Dependencies are inferred from the claim text.