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US 2015/0030544 A1Published application
Enzymatic Nanosensor Compositions and Methods
AssigneeNortheastern University
InventorsHeather A. Clark, Kevin Joseph Cash
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Abstract
Disclosed herein are compositions including a nanosensor that is sensitive to an analyte such that the nanosensor emits a fluorescent signal upon detecting the analyte, and a catalytic agent that catalyzes a reaction in which a target substrate is converted into one or more products, such that at least one of the one or more products is the analyte. In addition, methods of using the nanosensor-catalytic agent compositions to detect a target substrate are disclosed.
Claims28
A composition comprising:
- a catalytic agent that catalyzes a reaction in which a target substrate and/or a co-substrate is converted into one or more products;
- and
- a nanosensor that is sensitive to an analyte such that the nanosensor emits a fluorescent signal upon detecting the analyte, wherein the analyte is the target substrate, the co-substrate, or at least one of the one or more products.
A method of detecting a target substrate, comprising:
- (a) contacting a catalytic agent with a target substrate and/or a co-substrate such that the catalytic agent catalyzes conversion of the target substrate and/or the co-substrate into one or more products;
- (b) contacting a nanosensor with an analyte such that the nanosensor emits a fluorescent signal upon detecting the analyte, wherein the analyte is the target substrate, the co-substrate, or at least one of the one or more products;
- and
- (c) measuring the concentration of the target substrate based on the fluorescent signal generated by the nanosensor.
1 more element
Dependencies are inferred from the claim text.