Multianalyte molecular analysis using application-specific random particle arrays
AssigneeBioArray Solutions, Ltd.
InventorsSukanta BANERJEE, Michael Seul, Alice X. Li
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Abstract
The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interations of a number of analyte molecules in a sample.
Claims4
An encoded particle composition comprising
- a polystyrene bead with a diameter of between 0.2 and 200 microns
- wherein the bead is magnetic
- and is stained with two or more fluorophores,
- wherein the fluorophores are distinguishable by emission wavelength and
- generate an optical signature;
- and
- an oligonucleotide probe with a length of 15-25 bases
- wherein the sequence of the probe is selected to bind to a labeled amplicon generated from a genomic sample, and
- wherein the probe is attached to the bead via its 5′ terminus;
- and
- wherein the optical signature uniquely corresponds to the probe.
A method of forming a planar array of beads, comprising
- subjecting particles according to claim 62 to an electromagnetic field.
Dependencies are inferred from the claim text.