Measuring method for biological substance and measuring device therefor
AssigneePanasonic Corporation
InventorsTakaiki Nomura, Yasuyuki Nukina, Kazuhito Hato, Shuzo Tokumitsu
Life of this patent
Estimating the term…
Abstract
Provided is a measuring method for a biological substance, a measuring chip, and a measuring device which exhibit improved electrical responsiveness and reliability. For example, the device or chip provided for measuring the amount of a biological substance in a liquid being measured comprises immobilized antibodies, a substance that is labeled with an ion-conductive compound and that is bonded to the antibodies, and an electrode containing a working electrode and a counter electrode, and the working electrode has a thin film on the surface thereof that contains a hydrocarbon group.
Claims17
1-15. (canceled)
A chip for measuring the amount of a biological substance present in a liquid to be measured, comprising
- (a) an immobilized antibody;
- (b) a substance labeled with an ion conductive compound wherein the substance binds to the immobilized antibody;
- and
- (c) electrodes comprising a working electrode and a counter electrode, wherein the working electrode has, on its surface, a thin film comprising a hydrocarbon group and the number of carbon atoms of the hydrocarbon group is from 3 to 12, or
- (a′) an immobilized substance;
- (b′) an antibody labeled with an ion conductive compound wherein the antibody binds to the immobilized substance;
- and
- (c′) electrodes comprising a working electrode and a counter electrode, wherein the working electrode has, on its surface, a thin film comprising a hydrocarbon group and the number of carbon atoms of the hydrocarbon group is from 3 to 12.
A device for measuring the concentration of a substance, comprising
- an ion conductive compound with which a substance is labeled for measuring the amount of the substance in a liquid to be measured;
- electrodes comprising a working electrode having a surface on which a thin film containing a hydrocarbon group is formed, wherein the thin film-formed surface is a surface onto which the ion conductive compound is allowed to be adsorbed and the number of carbon atoms of the hydrocarbon group is from 3 to 12;
- an electric conductivity detecting section for detecting the electric conductivity of the thin film that is changed when an ion conductive compound moiety of the substance labeled with the ion conductive compound is adsorbed onto the working electrode;
- and
- a substance concentration calculating section for calculating the amount of the substance in the liquid to be measured based on a change in the electric conductivity.
A method for measuring the amount of a biological substance in a liquid to be measured, comprising
- (a) allowing an immobilized antibody to which a substance labeled with an ion conductive compound binds to contact with the biological substance in the liquid to be measured;
- (b) allowing an ion conductive compound moiety of the substance labeled with the ion conductive compound which is released by a replacement reaction between the biological substance and the substance labeled with the ion conductive compound to be adsorbed onto a thin film formed on a surface of an electrode and containing a hydrocarbon group, wherein the number of carbon atoms of the hydrocarbon group is from 3 to 12;
- and
- (c) measuring a change in the electric conductivity of the thin film, or
- (a′) allowing an antibody labeled with an ion conductive compound to contact with the biological substance in the liquid to be measured, wherein the antibody binds to an immobilized substance;
- (b′) allowing an ion conductive compound moiety of the antibody labeled with the ion conductive compound which is released by a replacement reaction between the biological substance and the antibody labeled with the ion conductive compound to be adsorbed onto a thin film formed on a surface of an electrode and containing a hydrocarbon group, wherein the number of carbon atoms of the hydrocarbon group is from 3 to 12;
- and
- (c′) measuring a change in the electric conductivity of the thin film.
Dependencies are inferred from the claim text.