Lipid arrays on fabricated devices and uses thereof

A method of simultaneously photobleaching fluorescent reagents located in a plurality of lipid bilayers and subsequently simultaneously monitoring the recovery of fluorescence by photobleaching through one face of an arraying substrate while monitoring fluorescence recovery on an opposite face of said substrate, said method comprising:

• providing an arraying substrate containing a plurality of magnetizable magnet elements that have a predetermined preferential axis of magnetization;
• providing a plurality of microdevices, each microdevice comprising a substrate, a FRAP Mask, an optical encoding pattern, and a patterned magnetic material wherein the patterned magnetic material has a predetermined preferential axis of magnetization, and wherein the microdevices are located at specific predetermined locations on the arraying substrate;
• providing a plurality of lipid bilayer regions wherein each bilayer region is localized on an individual microdevice;
• providing a fluorescent reagent present in the bilayer region;
• providing a high intensity light source and optics directed towards one face of the arraying substrate
• providing an excitation source and fluorescence monitoring system on the face of the arraying substrate opposite to the high intensity light source;
• and
• photobleaching said fluorescent reagent and monitoring the fluorescence recovery on said fluorescence monitoring system.

A system for simultaneously photobleaching fluorescent reagents located on a plurality of microdevices in an array in which fluorescent reagents are photobleached by directing light through one face of the arraying substrate and fluorescence recovery is subsequently simultaneously monitored on the opposite face of said substrate, said system comprising:

• an arraying substrate containing a plurality of magnetizable magnet elements that have a predetermined preferential axis of magnetization;
• microdevices comprising a substrate, a FRAP Mask, an optical encoding pattern, and a patterned magnetic material wherein the patterned magnetic material has a predetermined preferential axis of magnetization;
• and wherein the microdevices are located at specific predetermined locations on the arraying substrate;
• a plurality of lipid bilayer regions wherein each bilayer region is localized on an individual microdevice;
• a fluorescent reagent present in the bilayer region;
• a high intensity light source and optics directed towards one face of the arraying substrate;
• and
• an excitation source and fluorescence monitoring system on the face of the arraying substrate opposite to the high intensity light source.