Method for producing a population of oligonucleotides that has reduced synthesis errors
OwnerDerek Lee Lindstrom, Jeffrey R. Sampson +1inventors
InventorsDerek Lee Lindstrom, Jeffrey R. Sampson, Daniel E. Ryan
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Abstract
Provided herein is a method for producing a population of oligonucleotides that has reduced synthesis errors. In certain embodiments, the method comprises: a) obtaining an initial population of hairpin oligonucleotide molecules that each comprise a double-stranded stem region and a loop region; b) contacting the double-stranded region of the hairpin oligonucleotide molecules with a mismatch binding protein; and c) eliminating any molecules that bind to the mismatch binding protein, thereby producing a population of oligonucleotides that has reduced synthesis errors. A kit and a composition for performing the method are also provided.
Claims20
A method for producing a population of oligonucleotides that has reduced synthesis errors, comprising:
- a) obtaining an initial population of hairpin oligonucleotide molecules that each comprise a double-stranded stem region and a loop region;
- b) contacting the double-stranded region of said hairpin oligonucleotide molecules with a mismatch binding protein;
- and
- c) eliminating any molecules that bind to said mismatch binding protein, thereby producing a population of oligonucleotides that has reduced synthesis errors.
A kit comprising:
- a) a population of hairpin oligonucleotide molecules that each comprise a double-stranded stem region and a loop region;
- and
- b) a mismatch binding protein.
A composition comprising:
- a) a population of hairpin oligonucleotide molecules that each comprise a double-stranded stem region and a loop region;
- and
- b) a mismatch binding protein;
- wherein the mismatch binding protein is bound to any hairpin oligonucleotide molecules that have a synthesis error in the double-stranded stem region.
Dependencies are inferred from the claim text.