Disruption of CKX3 and at least one other CKX gene in a plant or plant cell leads to improved traits
OwnerThomas SCHMÜLLING, Isabel BARTRINA Y MANNS +1inventors
InventorsThomas SCHMÜLLING, Isabel BARTRINA Y MANNS, Tomás WERNER
Life of this patent
Estimating the term…
Abstract
The present invention is directed to isolated plant cells and transgenic plants comprising a disruption in at least a CKX3 gene and in one further gene encoding for a cytokininoxidase/dehydrogenase and being different from CKX3 well as to methods of producing such transgenic plants and to methods of increasing seed yield in a plant and/or plant height.
Claims26
A plant cell comprising a disruption in at least:
- i) an endogenous CKX3 gene encoding for a cytokinin oxidase/dehydrogenase comprising a polypeptide sequence being identical to or having at least 95% identity with SEQ ID No. 1 or an orthologous gene thereof;
- and
- ii) one further endogenous gene encoding for a cytokinin oxidase/dehydrogenase and being different from the gene defined in i);
- wherein said disruptions inhibit expression and/or activity of a product of the at least two disrupted cytokinin oxidase/dehydrogenase genes compared to a corresponding control plant cell lacking such disruptions.
A plant comprising a disruption in at least:
- i) an endogenous CKX3 gene encoding for a cytokinin oxidase/dehydrogenase comprising a polypeptide sequence being identical to or having at least 95% identity with SEQ ID No. 1 or an orthologous gene thereof;
- and
- ii) one further endogenous gene encoding for a cytokinin oxidase/dehydrogenase and being different from the gene defined in i);
- wherein said disruptions inhibit expression and/or activity of a product of the at least two disrupted cytokinin oxidase/dehydrogenase genes compared to a corresponding control plant lacking such disruptions.
A method of increasing seed yield in a plant and/or increasing plant height and/or increasing stem thickness relative to a corresponding control plant, the method comprising introducing in a plant a disruption in at least:
- i) an endogenous CKX3 gene encoding for a cytokinin oxidase/dehydrogenase comprising a polypeptide sequence being identical to or having at least 95% identity with SEQ ID No. 1 or an orthologous gene thereof;
- and
- ii) one further endogenous gene encoding for a cytokinin oxidase/dehydrogenase and being different from the gene defined in i);
- wherein said disruptions inhibit expression and/or activity of a product of the at least two disrupted cytokinin oxidase/dehydrogenase genes compared to a corresponding control plant lacking such disruptions.
A method for producing a plant with increased seed yield and/or plant height relative to a corresponding control plant, comprising disrupting in a plant at least:
- i) an endogenous CKX3 gene encoding for a cytokinin oxidase/dehydrogenase comprising a polypeptide sequence being identical to or having at least 95% identity with SEQ ID No. 1 or an orthologous gene thereof;
- and
- ii) one further endogenous gene encoding for a cytokinin oxidase/dehydrogenase and being different from the gene defined in i);
- wherein said disruptions inhibit expression and/or activity of a product of the at least two disrupted cytokinin oxidase/dehydrogenase genes compared to a corresponding control plant lacking such disruptions.
Dependencies are inferred from the claim text.