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US 2014/0373199 A1Published application
Polypeptides Having Beta-Xylosidase Activity and Polynucleotides Encoding Same
AssigneeNovozymes, Inc.
InventorsYu Zhang, Ye Liu, Junxin Duan, Lan Tang, Brett McBrayer
Life of this patent
Estimating the term…
Abstract
Provided are isolated polypeptides having beta-xylosidase activity and polynucleotides encoding the polypeptides. Also provided are nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
Claims18
1-17. (canceled)
An isolated polypeptide having beta-xylosidase activity, selected from the group consisting of:
- (a) a polypeptide having at least 60% sequence identity to the mature polypeptide of SEQ ID NO:
- 6 or SEQ ID NO:
- 8;
- at least 65% sequence identity to the mature polypeptide of SEQ ID NO:
- 2;
- or at least 75% sequence identity to the mature polypeptide of SEQ ID NO:
- 4 or SEQ ID NO:
- 10;
- (b) a polypeptide encoded by a polynucleotide that hybridizes under at least medium-high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, SEQ ID NO:
- 7, or SEQ ID NO:
- 9, (ii) the cDNA sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:
- 5, or SEQ ID NO:
- 7, or (iii) the full-length complement of (i) or (ii);
- (c) a polypeptide encoded by a polynucleotide having at least 60% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 5 or SEQ ID NO:
- 7, or the cDNA sequences thereof;
- at least 65% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 1 or the cDNA sequence thereof;
- or at least 75% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
- 3 or the cDNA sequence thereof or SEQ ID NO:
- 9;
- (d) a variant of the mature polypeptide of SEQ ID NO:
- 2, SEQ ID NO:
- 4, SEQ ID NO:
- 6, SEQ ID NO:
- 8, or SEQ ID NO:
- 10 comprising a substitution, deletion, and/or insertion at one or more (e.g., several) positions;
- and
- (e) a fragment of the polypeptide of (a), (b), (c), or (d) that has beta-xylosidase activity.
31 more elements
An isolated polynucleotide encoding a signal peptide comprising or consisting of amino acids 1 to 19 of SEQ ID NO:
- 2, amino acids 1 to 19 of SEQ ID NO:
- 4, amino acids 1 to 19 of SEQ ID NO:
- 6, amino acids 1 to 21 of SEQ ID NO:
- 8, or amino acids 1 to 20 of SEQ ID NO:
- 10.
2 more elements
Dependencies are inferred from the claim text.