Insulin derivatives Containing Additional Disulfide bonds
AssigneeNovo Nordisk A/S
InventorsPeter Madsen, Frantisek Hubalek, Thomas Boerglum Kjeldsen, Svend Ludvigsen
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Abstract
The present invention is related to insulin derivatives containing additional disulfide bonds and methods of making such.
Claims15
An insulin derivative having two or more cysteine substitutions and a side-chain attached to the insulin, where the three disulfide bonds of human insulin are retained, and the sites of cysteine substitutions are chosen in such a way that the introduced cysteine residues are placed in the three dimensional structure of the folded insulin derivative to allow for the formation of one or more additional disulfide bonds not present in human insulin.
A method for stabilizing an insulin comprising substituting two or more amino acids of an insulin with cysteine residues and attaching a side-chain to the insulin, wherein
- a. the three disulfide bonds of human insulin are retained and
- b. the sites of cysteine substitutions are chosen in such a way that the introduced cysteine residues are placed in the three dimensional structure of the folded insulin derivative to allow for the formation of one or more additional disulfide bonds not present in human insulin,
- thereby creating an insulin derivative comprising one or more additional disulfide bonds not present in human insulin.
Dependencies are inferred from the claim text.