Growth hormone polypeptides and methods of making and using same
AssigneeAmunix Operating Inc.
InventorsVolker Schellenberger, Joshua Silverman, Willem P. Stemmer, Chia-wei Wang, Nathan Geething, Jeffrey L. Cleland
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Abstract
The present invention relates to compositions comprising growth hormone linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in treatment of growth hormone-related diseases, disorders, and conditions.
Claims47
An isolated fusion protein, comprising a growth hormone (GH) sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO:
- 1, wherein said growth hormone is linked to an extended recombinant polypeptide (XTEN) of at least about 200 amino acids residues, wherein the XTEN is characterized in that:
- (a) the XTEN sequence comprises at least about 200 contiguous amino acids that exhibits at least 90% sequence identity to a comparable length of an amino acid sequence selected from Table 3;
- (b) the XTEN sequence lacks a predicted T-cell epitope when analyzed by TEPITOPE algorithm, wherein the TEPITOPE algorithm prediction for epitopes within the XTEN sequence is based on a score of −9 or greater;
- (c) it has a subsequence score of less than 10;
- and
- (d) the sum of glycine (G), alanine (A), serine (S), threonine (T), glutamate (E) and proline (P) residues constitutes more than about 90% of the total amino acid residues of the XTEN.
A fusion protein comprising an amino acid sequence having at least 90% sequence identity to the sequence of SEQ ID NO:
- 1 linked to an extended recombinant polypeptide (XTEN) sequence having at least 90% sequence identity to the sequence of SEQ ID NO:
- 75.
Dependencies are inferred from the claim text.