Programmable Arrays
AssigneesArizona Board of Regents, a body corporate of the State of Arizona, acting for and on behalf of, Engineering Arts LLC
InventorsPeter Wiktor, Joshua Labaer, Peter Kahn, Bharath Takulapalli, Ji Qiu, Al Brunner, Mitch Magee
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Abstract
Biomolecule arrays on a substrate are described which contain a plurality of biomolecules, such as coding nucleic acids and/or isolated polypeptides, at a plurality of discrete, isolated, locations. The arrays can be used, for example, in high throughput genomics and proteomics for specific uses including, but not limited molecular diagnostics for early detection, diagnosis, treatment, prognosis, monitoring clinical response, and protein crystallography.
Claims36
A biomolecule array, comprising
- (a) a first substrate;
- and
- (b) biomolecules comprising at least 10 isolated coding nucleic acids and/or at least 10 isolated polypeptides, wherein each nucleic acid and/or polypeptide is physically confined at a discrete location on the first substrate, and wherein each nucleic acid is capable of expressing its encoded product in situ at its discrete location on the substrate, and/or wherein each polypeptide is capable of a characteristic activity in situ at its discrete location on the substrate;
- wherein the discrete locations are separated from each other on the first substrate by a center to center spacing of between about 20 nm and about 1 mm.
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An array comprising
- (a) a silicon-containing substrate, having a surface comprising a plurality of nanowells at discrete locations wherein the discrete locations are separated from each other on the silicon-containing substrate by a center to center spacing of between about 20 nm and about 1 mm;
- and
- (b) biomolecules capable of capturing another molecule located at each discrete location.
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A method for expressing and capturing a product, comprising
- (a) contacting a nucleic acid array with reagents for nucleic acid expression to form a mixture, wherein the nucleic acid array comprises a first substrate and at least 10 isolated coding nucleic acids physically confined at a discrete location on a first substrate, and wherein each nucleic acid is capable of expressing its encoded product in situ at its discrete location on the first substrate;
- (b) optionally, bringing the first substrate into the proximity of a capture substrate;
- (c) incubating the mixture under conditions suitable for production of nucleic acid expression products;
- and
- (d) capturing and isolating the expression products on the first substrate or, when present, the capture substrate.
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Dependencies are inferred from the claim text.