Method and kit for determining- antibody sensitivity and clone cell strain
AssigneeShenzhen Mindray Bio-Medical Electronics Co., Ltd.
InventorsYumei Zhao, Lifang Dong, Ting Lei
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Abstract
A method and kit for determining antibody sensitivity and quality of a clone cell stain. The method comprises: obtaining a solid-phase carrier, cells and an antibody; adsorbing the antibody on the solid-phase carrier; incubating the cells and the antibody; preserving cells bound with the antibody; and dyeing and counting the cells bound with the antibody, so as to determine the antibody sensitivity or the quality of the clone cell stain based on the cell count. The kit comprises components used in the method.
Claims23
A method for determining antibody sensitivity, comprising:
- obtaining a solid-phase carrier, cells and an antibody;
- adsorbing the antibody on the solid-phase carrier;
- incubating the cells and the antibody;
- preserving cells bound with the antibody;
- dyeing and counting the cells bound with the antibody;
- and
- determining the antibody sensitivity based on the cell count.
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A method for determining antibody specificity, said method comprising:
- obtaining a solid-phase carrier, cells and an antibody, the cells including at least two different known types cells;
- adsorbing the antibody on the solid-phase carrier;
- incubating the cells and the antibody adsorbed on the solid-phase carrier;
- preserving cells bound with the antibody;
- dyeing and counting the cells bound with the antibody;
- and
- determining the antibody specificity based on the cell count of the two different known type cells.
A method for determining clone cell strain, comprising:
- providing a solid-phase carrier, cells and an antibody secreted by a clone cell strain;
- adsorbing the antibody secreted by the clone cell strain on the solid-phase carrier;
- incubating the cells and the antibody;
- preserving cells bound with the antibody;
- dyeing and counting the cells bound with the antibody;
- and
- determining the clone cell strain based on the cell count.
Dependencies are inferred from the claim text.