T cell receptor display

A diverse library comprising a plurality of phage particles displaying on their surfaces dimeric T cell receptor (TCR) polypeptide pairs constituted by:

• a TCR α chain variable domain sequence fused to the N terminus of a TCR α chain constant domain extracellular sequence;
• and
• a TCR β chain variable domain sequence fused to the N terminus of a TCR β chain constant domain extracellular sequence,
• wherein the TCR α chain constant domain extracellular sequence and the TCR β chain constant domain extracellular sequence are linked by a disulfide bond between cysteine residues substituted for:
• Thr 48 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 1 and Ser 57 of exon 1 of TRBC1*01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 5;
• Thr 45 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 2 and Ser 77 of exon 1 of TRBC1*01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 6;
• Tyr 10 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 3 and Ser 17 of exon 1 of TRBC1*01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 7;
• Thr 45 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 2 and Asp 59 of exon 1 of TRBC1*01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 8;
• or
• Ser 15 of exon 1 of TRAC*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 4 and Glu 15 of exon 1 of TRBC1*01 or TRBC2*01 at position 10 with reference to the position numbering of SEQ ID NO:
• 9.