Systems, apparatus and methods for biochemical analysis
AssigneeCelula, Inc.
InventorsKeunho Ahn, Benjamin Lai, Andree J. Pyfer, Yi Zhang, Haichuan Zhang
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Abstract
Systems, apparatus and methods are provided for biochemical analysis of a sample (e.g., a cell or nucleic acids). Samples are analyzed for molecular information and remain accessible for subsequent analysis or testing. The systems, apparatus and methods and systems provided are useful for performing quantitative and highly parallel biochemical reactions on biological samples in a high-throughput manner.
Claims22
An integrated system for performing a biochemical reaction on a sample comprising:
- a. a reaction module comprising:
- a device comprising a plurality of discrete reaction volumes, said reaction volumes comprising a reagent and a sample, wherein said reaction volume is accessible to perform more than one biochemical reaction on said sample;
- b. a dispensing module for dispensing said sample in the device, wherein said dispensing module can be operably coupled with said device;
- c. a thermal module comprising a heating element and a thermal control element, wherein said thermal module can be operably coupled to said device;
- d. a detection module to detect a signal from the discrete reaction volumes;
- e. a recovery module operable to individually recover at least a portion of or substantially all reacted material from each of said plurality of discrete reaction volumes, wherein said recovery module can be operably coupled with the device.
A method for analyzing a sample, the method comprising:
- a. dispensing said sample and a reagent into a plurality of discrete reaction volumes;
- b. performing a biochemical reaction on said discrete reaction volumes;
- c. detecting a signal from said discrete reaction volumes;
- and
- d. recovering at least a portion of or substantially all reacted material from said discrete reaction volumes.
An apparatus for receiving a device, the apparatus comprising:
- a base surface and a top surface and sidewalls including at least one opening for receiving said device;
- a pair of spaced guide rails for contacting said device to said base and preventing contact between said device and said top surface;
- said apparatus configured to receive a fluid.
Dependencies are inferred from the claim text.