US 2014/0370618 A1 · NUCLEIC ACID MOLECULE HAVING BINDING AFFINITY TO RODENT-DERIVED IGG ANTIBODY, BINDER, DETECTION REAGENT, AND DETECTION KIT

Abstract

The present invention is to provide a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the present invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 μM or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the present invention includes the binder for a rodent-derived IgG antibody of the present invention. The detection kit for detecting a rodent-derived IgG antibody of the present invention includes the detection reagent for detecting a rodent-derived IgG antibody of the present invention.

Claims14

Dependent claims

A nucleic acid molecule having a specific binding affinity to a mouse-derived IgG antibody and having a dissociation constant of 1 μM or less, wherein the nucleic acid molecule is a single-stranded nucleic acid represented by the general formula (I):

wherein, in said general formula (I), each of N1, N2, N3, N4, N5, N6, N7, and N8 indicates a nucleotide residue;
n1, n2, n3, n4, n5, n6, n7, and n8 respectively indicate the numbers of the nucleotide residues N1, N2, N3, N4, N5, N6, N7, and N8;
each of N1, N3, N5, and N7 form a loop structure;
N2 and N8 form a stem structure by binding to each other through hydrogen bonding;
and
N4 and N6 form a stem structure by binding to each other through hydrogen bonding.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

14/336,723Filed 21 Jul 2014

Priority

27 Sep 2012

Published

18 Dec 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification6 codes

G01N 33/6854
Involving proteins, peptides or amino acids: immunoglobulins
C12N 15/115
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: aptamers, i.e. nucleic acids binding a…
G01N 33/5308
Immunoassay; Biospecific binding assay; Materials therefor: for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
C12N 2310/16
Type of nucleic acid: aptamers

Nucleic acid molecule having binding affinity to rodent-derived igg antibody, binder, detection reagent, and detection kit

Life of this patent

Nucleic acid molecule having binding affinity to rodent-derived igg antibody, binder, detection reagent, and detection kit

Life of this patent

Abstract

Claims14