Lateral flow immunoassay for detecting vitamins
AssigneeNanospeed Diagnostics Inc.
InventorsRajan Gupta, Seema Gupta
Life of this patent
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Abstract
The invention is directed to a method for detecting analytes, particularly vitamins, using a lateral flow immunoassay. The method may be used to detect vitamin D, particularly 25-hydroxy vitamin D3 or 1,25-dihydroxy vitamin D3. The method involves obtaining a fluid sample from a subject; applying the sample to a lateral flow test strip comprising a conjugate pad capable of releasing a labelled antibody against vitamin D, and a detection membrane comprising a first capture reagent specific to the antibody and immobilized on a test band; and detecting the presence or absence of vitamin D in the sample. The presence of a detectable signal in the test band is indicative of vitamin D deficiency.
Claims19
A method of screening a fluid sample for a threshold value of vitamin D, comprising the steps of:
- (a) obtaining a fluid sample from a subject;
- (b) applying the sample to a lateral flow test strip comprising a conjugate pad comprising a known quantity of a labeled antibody against vitamin D;
- (c) allowing an immunocomplex to form between the labeled antibody and any vitamin D in the sample;
- (d) flowing the labeled antibody or immunocomplex through a test band comprising an immobilized first capture reagent capable of binding to:
- i. the labeled antibody but not the immunocomplex, or
- ii. the immunocomplex but not the labeled antibody;
- and
- (e) determining whether or not the amount of vitamin D in the sample exceeds a threshold value by detecting or not detecting the labeled antibody in the test band.
The method of 1, wherein the antibody is labeled with a label selected from a chromogen, a catalyst, a fluorescent compound, a chemiluminescent compound, a colloidal gold, a dye particle, or a latex particle tagged with a detector reagent.
The method of 7, wherein the label comprises colloidal gold.
A lateral flow immunoassay comprising a lateral flow test strip comprising a conjugate pad capable of releasing a labelled antibody against vitamin D, whereby the labeled antibody and any sample vitamin D forms an immunocomplex, and a detection membrane comprising an immobilized capture reagent capable of binding to i. the labeled antibody but not the immunocomplex, or ii. the immunocomplex but not the labeled antibody.
Dependencies are inferred from the claim text.