Trophectodermal cell-specific gene transfer methods

A method for introducing a polynucleotide specifically into a trophectodermal cell, which comprises the steps of:

• a) extracting a blastocyst from an animal;
• b) removing zona pellucida from the blastocyst;
• and
• c) introducing a polynucleotide into trophectodermal cells of the blastocyst by placing the blastocyst comprising trophectodermal cells obtained in step (b) in a solution comprising viral vectors;
• wherein the viral vectors introduce the polynucleotide only into the trophectodermal cells of the blastocyst.

A method for introducing a polynucleotide specifically into a trophectodermal cell, which comprises the steps of:

• a) extracting a blastocyst from an animal;
• b) removing zona pellucida from the blastocyst;
• and
• c) introducing a polynucleotide into trophectodermal cells of the blastocyst with a nucleic acid transfection reagent;
• wherein the polynucleotide are introduced only into the trophectodermal cells of the blastocyst.

A method for introducing a polynucleotide specifically into a trophectodermal cell, which comprises the steps of:

• a) extracting a blastocyst from an animal;
• b) removing zona pellucida from the blastocyst;
• and
• c) using a viral vector to introduce a polynucleotide only into trophectodermal cells of the blastocyte, wherein the polynucleotide encodes a protein selected from the group consisting of Dlx3, Fgfr2, Fra1, Fzd5, Gab1, Gcm1, Grb2 hypomorph, Gja7, Hgf, Hsp84-1, Itgav, Junb, Lifr, ERK1, ERK2, ERK5, MEK1, MEKk3, p38α, p38β, Met, Pdgfra, Pdgfb, Pparg, Rxra, Rxrb, Sos1, Vhlh, Wnt2, Ets2, Mash2, Egfr, Hsf1, Bmp5, Bmp7, Dnmt1, Itga4, Lhx1, Mrj, Tcf, Lef, Cdx2, Eomes, Fgf4, Esrrb, Hand1, Mdfi, Esx1, Arnt, Tcfeb, and Gjb2;
• wherein the viral vectors introduce the polynucleotide only into the trophectodermal cells of the blastocyst.