Method for preparing biological tissues for use in biological prostheses
AssigneeSorin Group Italia S.r.l.
InventorsMarina Strasly, Giovanni Rolando
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Abstract
A method of treating a biological tissue for biological prostheses includes steps of fixation of the biological tissue via a fixing solution including glutaraldehyde and detoxification of the fixed biological tissue via treatment with a detoxifying solution. The detoxification step includes one or both of eliminating phospholipids via treatment with an elimination solution and a treatment with a detoxifying solution. The elimination solution includes 1,2-octanediol and ethanol. The detoxifying solution includes taurine or homocysteic acid.
Claims20
A method of treating a biological tissue for use in a biological prosthesis, the method comprising the steps:
- obtaining biological tissue;
- treating the biological tissue using an elimination solution to eliminate phospholipids from the biological tissue, the elimination solution comprising 1,2-octanediol at a concentration range of 1% to 25% v/v and ethanol at a concentration range of 25% to 49% v/v;
- and
- detoxifying the biological tissue using with a detoxifying solution at a temperature above room temperature, the detoxifying solution comprising taurine or homocysteic acid;
- wherein treatment of the biological tissue to eliminate the phospholipids precedes detoxification of the biological tissue.
The method according to claim 19, wherein the fixing solution includes glutaraldehyde at a concentration expressed as v/v in the range of 0.10% to 2.00%.
The method according to claim 19, wherein the fixing solution contains glutaraldehyde at a concentration expressed as v/v in the range of 0.30% to 1.00%.
Dependencies are inferred from the claim text.