Use of Synthetic Scaffolds for the Production of Biosynthetic Pathway Products
AssigneeThe Regents of the University of California
InventorsJohn E. Dueber, Jay D. Keasling, Gabriel C. Wu, Ghulam Reza K. Malmirchegini
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Abstract
The present invention provides methods of producing a product or product precursor of a biosynthetic pathway in a genetically modified host cell. The present invention also provides genetically modified host cells comprising nucleic acids encoding a scaffold polypeptide and nucleic acids comprising nucleotide sequences encoding two or more enzymes in a biosynthetic pathway. The present invention further provides nucleic acids comprising nucleotide sequences encoding scaffold polypeptides, for use in a subject method.
Claims16
-8. (canceled)
A nucleic acid comprising a nucleotide sequence encoding a scaffold polypeptide of the formula (X1)n1(Y1)(X2)n2(Y2)(X3)n3, wherein X1, X2, and X3 are different peptide binding elements, wherein each of n1, n2 and n3 is independently an integer from one to about 10, and wherein Y1 and Y2, if present, are linker peptides.
(canceled)
Dependencies are inferred from the claim text.