US 2014/0370574 A1 · Metabolically Enhanced Cyanobacterial Cell for the Production of Ethanol

Abstract

A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol. The invention also provides a method for producing the metabolically enhanced cyanobacterium, a method for producing ethanol with the metabolically enhanced cyanobacterium, and a method for screening of alcohol dehydrogenase enzyme expressing cyanobacterial strains for the presence of NADPH-dependent native alcohol dehydrogenase enzymes.

Claims25

Dependent claims

A metabolically enhanced cyanobacterial cell for the production of ethanol, comprising:

a. a recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and
b. a recombinant gene encoding a Zn2+ dependent alcohol dehydrogenase enzyme (Adh) converting acetaldehyde to ethanol, wherein the amino acid sequence of said Zn2+ dependent alcohol dehydrogenase enzyme (Adh) is at least 95% identical to the Adh amino acid sequence from an organism selected from the group consisting of Arthrospira platensis, Arthronema africanum, Chroococcidiopsis sp., Synechococcus sp., Lyngbya sp., Cyanothece sp., and Cyanobacterium sp.

Dependencies are inferred from the claim text.

Facts

Application

14/305,781Filed 16 Jun 2014

Priority

14 Jun 2013

Published

18 Dec 2014

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

C12P 7/065
Ethanol, i.e. non-beverage: with microorganisms other than yeasts
C12N 9/0006
Oxidoreductases: acting on CH-OH groups as donors
C12N 9/88
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: lyases
C12Y 101/01001
With NAD+ or NADP+ as acceptor: alcohol dehydrogenase

Metabolically Enhanced Cyanobacterial Cell for the Production of Ethanol

Life of this patent

Metabolically Enhanced Cyanobacterial Cell for the Production of Ethanol

Life of this patent

Abstract

Claims25